Northland College Women's Hockey Roster, These percentages are automatically calculated for each compound currently on the graph. Rua Almada Negreiros Lote 5, Loja 14 2615-275 Alverca do Ribatejo - Portugal Tel. IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, Shan, A protean clamp guides membrane targeting of tail-anchored proteins, Proceedings of the National Academy of Sciences 114, E8585-E8594 (2017). This model also has an integrated IPU and is operated via a compact LCD colour touchscreen. P. Zhan, M. Urban, S. Both, X. Duan, A. Kuzyk, T. Weiss, N. Liu, DNA-assembled nanoarchitectures with multiple components in regulated and coordinated motion, Science Advances 5, eaax6023 (2019). Chem. : +32 2 31 50 800 Fax: +32 2 31 50 801 E-mail: [email protected] Order anti-KCNQ1 anticorps ABIN2483166. When Did The Hamburger Become Popular, Levin, Antibodies to an Intracellular Antigen Penetrate Neuronal Cells and Cause Deleterious Effects, J. Clin. Through the use of various reporter stains, fluorescence-based . Under the "Export" submenu on the left side of the screen, the user may instantly download the spectrum as a .png file to their computer by clicking "Image" under the "Export" Menu on the left side of the window. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers. C. Kim, O.-c. Lee, J.-Y. Standard throughput mode can be selected for acquisition of larger sample volumes. A. Intracellular and Plasma Membrane Cholesterol Labeling and - PubMed M. Pagliaro, R. Ciriminna, M. Wong Chi Man, S. Campestrini, Better Chemistry through Ceramics: The Physical Bases of the Outstanding Chemistry of ORMOSIL, J. Phys. Second, it must collimate that light so that all rays propagate parallel to each other and can travel through the collection path without diverging. Flow cytometry laser line: 633, 635 or 640 nm Microscopy laser line: 633, 635 or 640 nm Direct replacement for: Cy5, Alexa Fluor 647 and ATTO 647N 0 20 40 60 80 100 0 20 40 60 80 100 450 500 550 600 650 700 750 800 850 Absorption Emission Wavelength(nm) CF640R Cy5 Figure 3. Chen, K. Chetal, G. Mantalas, N. Neff, E. Jabart, A. Sharma, G. Nolan, N. Salomonis, J. Wu. The flow ratio of sample to sheath was altered to try to confine the beads as narrowly as possible in the centre of the channel: a 1:10 sample to sheath ratio produced a 3 m wide stream for 2.5 m beads and 2:9 ratio produced a 6.5 m stream width for 6 m beads. Corrie, R. Vogel, I. It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta. The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. In search of new approaches to treat bone infection and prevent drug resistance development, a nanosystem based on hollow bioactive glass nanoparticles (HBGN) of composition 79.5SiO2-(18-x)CaO-2.5P2O5-xCuO (x = 0, 2.5 or 5 mol-% CuO) was developed. T. Munmun, A. Kabir, K. Sada, A. Kakugo, Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide, Sensors and Actuators B: Chemical 304, 127231 (2020). . Molinero-Fernndez, M. Moreno-Guzmn, L. Arruza, M. Lpez, A. Escarpa, Polymer-Based Micromotor Fluorescence Immunoassay for On-the-Move Sensitive Procalcitonin Determination in Very Low Birth Weight Infants Plasma, ACS Sensors 5, 1336 (2020). Galifornia Wholesale Phone Number, Upon arrival, it should be stored at -20C. endstream endobj 10 0 obj <>>> endobj 11 0 obj <> endobj 12 0 obj <>/Font<>/ProcSet[/PDF/Text/ImageC]/XObject<>>>/Rotate 0/TrimBox[0.0 0.0 1728.0 2592.0]/Type/Page>> endobj 13 0 obj <>stream ATTO dyes are characterized by strong absorption (high extinction coefficient), high fluorescence quantum yield, and high photo-stability. Spectra Analyzer - BioLegend These conjugates are ideal for imaging and other applications requiring increased sensitivity and environmentally insensitive fluorescence detection. Changing color contrast based on dark backgrounds Orai1 (also known as CRACM1) acts as the store-operated calcium channel (SOC) and STIM1 as the endoplasmic reticulum (ER) Ca2+sensor.3,4The majority of STIM1 appears to be localized intracellularly at the ER membrane while low expression of STIM1 has been detected on the cell surface of several cell types.5STIM1 has an amino-terminal EF hand Ca2+binding domain facing the lumen of the ER.6Upon Ca2+store depletion, STIM1 molecules are redistributed in punctae underneath the plasma membrane and activate SOCs. Herten, G. Nette, G. Schenk, M. Seefeld, Is CuII Coordinated to Patellamides inside Prochloron Cells?, Chemistry - A European Journal (2017). 0000038853 00000 n B. Agrawalla, T. Wang, A. Riegger, M. Domogalla, K. Steinbrink, T. Drfler, X. Chen, F. Boldt, M. Lamla, J. Michaelis, S. Kuan, T. Weil, Chemoselective Dual Labeling of Native and Recombinant Proteins, Bioconjugate Chemistry 29, 29 (2018). Click "Show Crosshairs" under the "Analyze" submenu in order to trace the exact curve of the currently-selected compounds" fluorescent intensity across the horizontal axis. K. Banas, N. Rivera-Torres, P. Bialk, B.-C. Yoo, E. Kmiec, Kinetics of Nuclear Uptake and Site-Specific DNA Cleavage during CRISPR-Directed Gene Editing in Solid Tumor Cells, Molecular cancer research : MCR 18, 891 (2020). Antibodies allow selective detection of specific proteins. D. Rutz, Q. Luo, L. Freiburger, T. Madl, V. Kaila, M. Sattler, J. Buchner. Cell cycle progression was investigated by flow cytometry of DNA content. P.P.T. startxref 0 0000270695 00000 n 0000276030 00000 n Long, K. Ubych, E. Jagu, R. Neely, FRET-Based Method for Direct, Real-Time Measurement of DNA Methyltransferase Activity, Bioconjugate Chemistry 32, 192 (2021). Fridrikh, Staphylococcus aureus Strain Typing by Single-Molecule DNA Mapping in Fluidic Microchips with Fluorescent Tags, Clinic. Atto 550 is a fluorescent compound with an excitation peak at 554 nm and an emission peak at 575 nm. SPIE 8590, 85900D (2013). 0000186769 00000 n The outcomes of flow cytometry have shown that PDGFR-positive cells shared similar CD140 profile with fibroblasts as indicated by the similarity in their cell count and mean fluorescence intensity (Figure 6A,B). Up to 14 parameters from 4 lasers Our reagent specialists are here to help you find the best product for your application. Starbound Weapon Tiers, 0000196280 00000 n M. Pazos, K. Peters, M. Casanova, P. Palacios, M. VanNieuwenhze, E. Breukink, M. Vicente, W. Vollmer, Z-ring membrane anchors associate with cell wall synthases to initiate bacterial cell division, Nature Communications 9, 5090 (2018). The fluorescence is excited most efficiently in the range 575 610 nm. Syeda Rubaiya Nasrin, Arif Md. We aim to ensure that digital services are accessible to people with disabilities, and therefore we have invested large resources in order to simplify the use of the website for people with disabilities as much as possible, out of the belief that every person deserves the right to live with equality, dignity, convenience, and independence. Tetramethylrhodamine (TRITC) | Thermo Fisher Scientific - US Low carryover is essential in research applications to ensure sample purity and data integrity. Atto 647N is an extraordinary highly fluorescent dye, and Atto 655 are alternatives to Cy5 and Alexa Fluor 647. Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. B 117, 11932 (2013). J. Strmqvist, L. Nardo et al., Binding of Biotin to Streptavidin: A combined fluorescence correlation spectroscopy and time-resolved fluorescence study, Eur. 0000000016 00000 n PDF Fluorophore and reagent selection guide for flow cytometry The objective of the study was to evaluate the capacity of the HBGN to be used as a nanocarrier of the broad-spectrum antibiotic danofloxacin and . ATTO 550 Orange 554 576 791 Cy3.5 Orange Red 581 596 1,286 microscopy, flow cytometry and immunohistochemistry. Victoria Power Station, US Patent for Method for the site-specific enzymatic labelling of Untreated GPE86 cells serve as control (ctrl). View Technology. Note: If a filter is added to the graph, a new column will appear in the information table at the bottom of the page, labeled "Spillover" with the filter shown in parentheses. Northland College Women's Hockey Roster, Please activate JavaScript to have access to all shop functions and all shop content. It allows simultaneous multi-parameter analysis of single cells. . 0000002715 00000 n To add one or more excitation sources, click "Excitation Source" in the "Add" submenu on the left part of the screen. M. Sauer, S. Juranek, J. 19% tax excl. When Did The Hamburger Become Popular, Tiny channels between nerve cells are involved in a newly discovered mechanism of how Parkinsons disease can spread throughout the brain, according to new research from Linkping University, Sweden.. Where indicated, 5 M GM6001 was incubated for 30 min at 37C with PMNs before DCFDA loading and stimulation. To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen. Reagent Selection Guide for the Attune Flow Cytometers Clicking on the menu opens accessibility buttons. As expected, the addition of the amphiphile triggered the appearance of fluorescent pixels in the red channel of the confocal fluorescent microscopy images (Fig. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594. 0000196464 00000 n ATTO-590. Merged image CF450 Dye A green fluorescent dye with unique spectral properties. 25, 2166 (2014). Whittle, Use of universal reporter primers in multiplex PCR of autosomal loci, FSI Genetics Suppl. Glass, N. Shi, L. Yang, M. Wang, Q. Xu, Intracellular delivery and biodistribution study of CRISPR/Cas9 ribonucleoprotein loaded bioreducible lipidoid nanoparticles, Biomaterials Science 7, 596 (2019). 0000213898 00000 n White, A. New developments in illumination sources, digital signal processing and microsphere chem. H. Mannell, J. Pircher et al., Targeted Endothelial Gene Delivery by Ultrasonic Destruction of Magnetic Microbubbles Carrying Lentiviral Vectors, Pharm. First, we report a robust method for quantifying plasma membrane cholesterol by flow cytometry using the GFP-D4 probe. Chem. 0 DC sheath flow detection method Sysmex analysers use the DC sheath flow detection method to count red blood cells and platelets, RBC and PLT. %%EOF Insulin and insulin-like growth factors (IGFs) act on tetrameric tyrosine kinase receptors controlling essential functions including growth, metabolism, reproduction and longevity. Immunol. Approximately 10,000 cells can be analyzed and processed by a computer in less than one minute. ATTO-488. Chem. The results demonstrate that harmful protein aggregates, or deposits, can bind and hitch a lift with channel-forming proteins, and in this way spread to healthy cells. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. Second, to optically distinguish and quantify intracellular cholesterol accumulation, we have adapted the classical filipin cholesterol staining protocol. BUV395 is designed for instruments equipped with a 355 nm UV laser and a 379/28 filter. 0000276406 00000 n Claude, A. Moreau, J. Lumeau, J. Wenger, Extending Single-Molecule Frster Resonance Energy Transfer (FRET) Range beyond 10 Nanometers in Zero-Mode Waveguides, ACS Nano 13, 8469 (2019). R. Roy, S. Hohng, T. Ha, A practical guide to single-molecule FRET, Nature Methods 5(6), 2008, 507-516. K. Gpfrich, M. Urban, C. Frey, I. Platzman, J. Spatz, N. Liu, Dynamic Actuation of DNA-Assembled Plasmonic Nanostructures in Microfluidic Cell-Sized Compartments, Nano letters 20, 1571 (2020). We offer 100% guarantee on all our products. A one parameter histogram plotting channel number vs. number of events. Commun. In a-PBTs, in addition to K + channel activity and Ca 2+ fluxes, chemotaxis was measured. Yang, E. Cooper, B. Chen, K. Siminovitch, A. Peterson, TIE, The CRISPR journal 1, 223 (2018). of lymphocytes and other cells, owing to its ability to make quant., homogeneous, multiparameter measurements of particles. 49913 - ET - 633-640nm Laser Longpass Set for AlexaFluor 647, DyLight 649, Atto 647: 49914 - ET - 640-647nm Laser Bandpass for Set AlexaFluor 647, DyLight647, Atto 647N: 49915 - ET - 355-375nm Laser Longpass Set for Uncaging and Ablation: 49916 - ET - Shortpass Filter Set for 1064nm Laser Tweezing or CARS Beam Combining By using the right combination of channel exposed 200ms, green channel exposed 800ms. Rep. 33, e00046 (2013). R. Schoch, I. Barel, F. Brown, G. Haran, Lipid diffusion in the distal and proximal leaflets of supported lipid bilayer membranes studied by single particle tracking, The Journal of Chemical Physics 148, 123333 (2018). 2023 BD. A dump channel will be created where you can add as many markers as is necessary. Lo, F. Emran, I. Kays, X.-J. Special Order Research Product (SORP) Program for Custom Solutions Email: [email protected], CopyRight 2018 CDL Technical & Motorcycle Driving School, Hours of Service (Log Books) 8 Hours Certification Course, CMV Driver Knowledge & Skills Evaluation 6 Hours Certificatrion Course, CDL 6 Hours Preparation Course Class B-Truck, P-Bus, S-Bus, CDL 10 Hours Preparation Course Class A, B-Truck, P-Bus, S-Bus, COURSES CDL 20 Hours Preparation Course Class A, B-Truck, P-Bus, S-Bus, Heavy Commercial 40 Hours CDL Class A Tractor Trailer Certification Course, COURSES Light Commercial 40 Hour CDL Class B\P-Bus, S-Bus Certification Course, CDL Class A 80 Hours Intermediate Tractor Trailer Certification Course, Bode Plot Solved Examples In Control System Pdf. The program is subject to the conditions of use of the manufacturer. Please fill in the following information and we will get in touch with you regarding S. Bultmann, R. Morbitzer et al., Targeted transcriptional activation of silent oct4 pluripotency gene by combining designer TALEs and inhibition of epigenetic modifiers, Nucl. Products Learn Support Quality About Us Contact Us Custom Solutions Custom Reagents Custom Services Custom Requests Form Login/Register (0) Menu Login/Register (0) During the process, a sample of cells or particles is suspended in fluid and injected into a flow cytometer machine. Terms of Payment. Fluorescence Spectrum Analyzer for Flow Cytometry Use this spectral viewer tool to compare fluorescent spectra excitation by different lasers and emission into different bandpass filters as an aid to multicolor flow cytometry panel design. Spectrum [Atto 550] | AAT Bioquest E. Favaro, D.R. StarBright Blue 580 Dye is a new, proprietary, fluorescent nanoparticle from Bio-Rad. Y. Cy is a trademark of Global Life Sciences Solutions Germany GmbH or an affiliate doing business as Cytiva. M. Barbiero, S. Castelletto, Q. Zhang, Y. Chen, M. Charnley, S. Russell, M. Gu, Nanoscale magnetic imaging enabled by nitrogen vacancy centres in nanodiamonds labelled by iron-oxide nanoparticles, Nanoscale 12, 8847 (2020). S. Lee, J.-H. Bong, J. Jung, J. View theBD LSRFortessa System brochure. I. Hoffecker, S. Chen, A. Gdin, A. Bosco, A. Teixeira, B. Hgberg, SolutionControlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019). 0000214115 00000 n A. Extracellular staining of cells with, CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, Key antibody specifications include clonality, reactivity, host, and conjugation. Maximum absorption 601 nm; Maximum fluorescence 627 nm. Chen, K. Chetal, G. Mantalas, N. Neff, E. Jabart, A. Sharma, G. Nolan, N. Salomonis, J. Wu, Defining human cardiac transcription factor hierarchies using integrated single-cell heterogeneity analysis, Nature Communications 9, 4906 (2018). Cytek Aurora [Spectral Flow Cytometry] | Cytek Biosciences 0000007840 00000 n 0000214142 00000 n Flow Cytometry: Rinse samples once in Incubation Buffer. Luke Summer House Ex Girlfriend, CDL Technical & Motorcycle Driving School %PDF-1.7 % She, R. Tornay, E. Leimgruber, D. Bernasconi, L. Lagopoulos, P. Renaud, N. Demierre, P. van den Bogaard, Rapid, sensitive and real-time multiplexing platform for the analysis of protein and nucleic-acid biomarkers, Analytical Chemistry 87, 1582 (2015). Shipping Information Protect from light. %PDF-1.4 % Lyophilized powder. Multiple sclerosis (MS) is the most frequent neurological disease that leads to premature retirement in young adults. The light produced by lasers in a flow cytometer is scattered by cells in the sample, measured by detectors, and then translated to signals that can be analyzed and measured. Contact our Technical and Applications Supportpersonnel for maintaining optimal instrument performance and with any other instrument-related support. Luke Summer House Ex Girlfriend, This page has been recently translated and is available in French now. U. Chio, S. Chung, S. Weiss, S.-O. 0000288376 00000 n Pw|riwc The fluorescence is excited most efficiently in the 540 - 565 nm range. ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B, the commercial alternative to NEDTM. Starbound Weapon Tiers, 0000007304 00000 n 0000006328 00000 n 0000002015 00000 n Spectra Viewer Select machine + Add Fluorophore Fluorophores Ex. A portion of blood is separated from the aspirated whole blood and mixed with the diluent in a pre-set ratio. B. Dalzon, C. Aude-Garcia, V. Collin-Faure, H. Diemer, D. Bal, F. Dussert, D. Fenel, G. Schoehn, S. Cianfrani, M. Carrire, T. Rabilloud, Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles, Nanoscale 9, 9641 (2017). Bode Plot Solved Examples In Control System Pdf, Alexa Fluor Atto 550-RX002 Atto 550-RX005 Atto 550-RX014 Atto 550-RX017 Atto 550-RX020 Atto 550-RX023 Atto 550-RX026 Atto 550-RX029 Atto 550-RX032 Atto 550-RX035 Atto 550-RX041 Quantification of cellular uptake: For peptideuptake studies by flow cytometry, cells were seeded in a 24well plate (HEK293 500 000, MCF7 200 000 cells per well) and grown to 7080 % confluency. About ATTO 550. Pharmaceutics | Free Full-Text | Cu-Doped Hollow Bioactive Glass Quantitative Comparison of Long-wavelength Alexa Fluor Dyes to Cy Dyes IJMS | Free Full-Text | Allosteric Inhibition of c-Abl to Induce This application claims benefit under 35 U.S.C. your query. D. Kozak, A. Chen, M. Trau, Profiling Protein-Surface Interactions of Multicomponent Suspensions via Flow Cytometry, Langmuir 24, 1204 (2008). Am. To detect far-red fluorescence in cells labeled with the Alexa Fluor 647 or Cy5 A set of polymer particles stained with at least two fluorescent dyes is presented. please visit our Contact Us page. It is commonly used in the following applications: Super Resolution Microscopy, Nucleic Acid Labeling, Flow Cytometry, FISH. Y. Li, A. Chukun Li, Q. Xu, Intracellular Delivery of HisTagged GenomeEditing Proteins Enabled by Nitrilotriacetic AcidContaining Lipidoid Nanoparticles, Advanced Healthcare Materials 8 (2019). Flow cytometry of human islet cells gomyelin, 10 mM in HDMEM; ATTO-tec, Germany) for 20 min on ice. Product Sheets PDF Flow Cytometry Resource How the flow cytometer is set up determines how the fluorophore is detected. Victoria Power Station, 0000191226 00000 n The octagon- and trigon-shaped optical pathways of collection optics maximize signal detection and increase sensitivity and resolution allowing you to identify dim and rare cell populations Can be configured with up to 5 lasersblue, red, violet, UV and yellow-green. Kang, J. Jose, J.-C. Pyun, Screening of biotin-binding FV-antibodies from autodisplayed FV-library on E. coli outer membrane, Analytica Chimica Acta 1169, 338627 (2021). Storage. D. Roth, M. Nasir, P. Ginzburg, P. Wang, A. 152, 170 (2010). Chem. At least 16 subsets of particles can be resolved on the basis of variable emission from the at least two fluorescent dyes where emission from at least one dye derives from a fluorescent dye covalently attached to the particle surface. All Rights Reserved. The fluorescence is excited most efficiently in the 540 - 565 nm range. 85, 7753 (2013). E. Ronzitti, B. Harke, A. Diaspro, Frequency dependent detection in a STED microscope using modulated excitation light, Optics Express 21, 201 (2013). If our products do not perform as stated, please contact our technical support staff. Despite our efforts to enable website browsing for all the website pages, there may be website pages that haven't been made accessible yet or may lack a suitable technical solution. Series 3, e71 (2011). Clicking on the menu opens accessibility buttons. 0000005723 00000 n After coupling to a substrate the dye carries a net electrical charge of +1. We showed that IGF-II induces cell proliferation and gene transcription when IR-B is over-expressed. Please fill in the following information and we will get in touch with you regarding your query. 0000075446 00000 n Galifornia Wholesale Phone Number, 0000003399 00000 n This may be done either as part of an infertility workup or to see if a vasectomy has been successful. Tel: +1 877 302 8632 Fax: +1 888 205 9894 (Toll-free) E-Mail: [email protected] Search results for ATTO Antibody at Sigma-Aldrich. Victoria Power Station, I. Haq, J.A. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers. - streptavidin J. Wardyn, A. Chan, A. Jeyasekharan, A Robust Protocol for CRISPR-Cas9 Gene Editing in Human Suspension Cell Lines, Current Protocols 1, e286 (2021). XN-550 - Products Detail Fluorescent ATTO Dyes | Alomone Labs ATTO 550 is a yellow emitting dye that can be excited by the 532 laser and emission captured by the 585/42 filter. Click "Hide Crosshairs" to return to the default. S. Baliga, C. Murphy, L. Sharon, S. Shenoy, D. Biranthabail, H. Weltman, S. Miller, R. Ramasamy, J. Shah, Rapid method for detecting and differentiating Mycobacterium tuberculosis complex and non-tuberculous mycobacteria in sputum by fluorescence in situ hybridization with DNA probes, International Journal of Infectious Diseases 75, 1 (2018). Syeda Rubaiya Nasrin, Tsukasa Ishihara, Arif Md. 0000286343 00000 n S. Chavan, D. Kim, J. Hwang, Y. Choi, J. Hong, J. Kim, M.-H. Lee, M. Hwang, J. Choi, Enhanced Detection of Infectious Pancreatic Necrosis Virus via Lateral Flow Chip and Fluorometric Biosensors Based on Self-Assembled Protein Nanoprobes, ACS Sensors 4, 2937 (2019). This flexibility in laser wavelengths allows assay design to be optimized using the latest fluorescent dyes and substrates. Rua Almada Negreiros Lote 5, Loja 14 2615-275 Alverca do Ribatejo - Portugal Tel. 42 0 obj <>/Filter/FlateDecode/ID[<9473BD190E70408FBB7CCF0FFC9676FA>]/Index[9 57]/Info 8 0 R/Length 144/Prev 546667/Root 10 0 R/Size 66/Type/XRef/W[1 3 1]>>stream Additional file 4: Figure S4 Internalization of FITC-Insulin and BAC-Ins-SA-atto 550. ATTO-594. P2Y receptors can be divided into two main subgroups: the P2Y 1 subfamily which comprises P2Y 1, P2Y 2, P2Y 4, P2Y 6, and P2Y 11 receptor subtypes. Flow cytometry is unique in its ability to measure, analyze, and study vast numbers of homogenous or heterogeneous cell populations.